Cell Signaling Technology
Bcl-2 (D17C4) Rabbit mAb (Mouse Preferred)
3498
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Our lab studies the role of mTOR signaling in T cell such as mTORC1 and mTORC2 signaling during T cell differentiation and function. In addition, mTOR signaling plays a role in cell survival. Therefore, we measured expression of the anti-apoptotic transcription factor Bcl2 with immunoblot analysis in our T cell lysates. This is a nice antibody with great specificity.
Western Blot
Activated murine CD8+ T cells
1:4000 in 4%BSA/TBST 4 deg overnight with shaking
5% dry milk/TBST 1 hr room temperature with shaking
1:5000 anti-Rabbit HRP (CST) in %5 milk/TBST 1 hr room temp with shaking
None
UVP Imaging System
We are able to detect robust protein expression of Bcl-2 in T cell lysates. The band also resides at the correct molecular weight size.
N/A
Robust antibody and good dilution. Can reuse diluted antibody many many times.
Awesome antibody to detect Bcl-2 expression in lysates.