Cell Signaling Technology
Bcl-xL (54H6) Rabbit mAb
2764
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Our lab studies the role of mTOR signaling in CD8 T cell differentiation and function. mTOR signaling controls the metabolic programming of T cells but is also very important in controlling cell survival to prevent apoptosis by increasing anti-apoptotic transcription factors. Therefore, we analyzed Bcl-xL protein expression in lysates.
Western Blot
Murine CD8+ T cells
1:3000 in 4%BSA/TBST 4 deg overnight with shaking
5% dry milk/TBST 1 hr room temperature with shaking
1:5000 anti-Rabbit HRP (CST) in %5 milk/TBST 1 hr room temp with shaking
None
UVP Imaging System
We are able to detect Bcl-xL protein expression in our lysates. Also, the band is similar to the reported bands on the product page at the appropriate molecular size level.
N/A
Great antibody at good dilution. Can reuse diluted antibody if stored properly.
None whatsoever
Great and reliable antibody to detect the anti-apoptotic transcription factor Bcl-xL in lysates for immunoblot analysis.