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APC anti-mouse CD366 (Tim-3) Antibody
119706
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Browse Similar Products: Tim-3 Flow Cytometry Antibodies
Our cancer immunology lab studies T cell exhaustion in the setting of cancer. TIM3 surface expression is what we routinely analyze on T cells to determine level of exhaustion; therefore, we use this flow antibody in our T cell exhaustion panel.
Flow Cytometry
Activated murine CD8+ T cells
1:250 in 2%FBS/PBS solution
100ng/mL FC Block before staining
None
BD FACS Celesta
We are readily able to identify TIM3 positive cells along with PD1 as it's been reported in the literature. This double positive population indicates extreme exhaustion.
N/A
One of the few antibodies on the market for reliable TIM3 detection.
Higher dilution needed otherwise none.
Great antibody to use for TIM3 surface detection especially when studying exhaustion.