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Brilliant Violet 785™anti-mouse CD223 (LAG-3) Antibody
125219
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As an immunology lab, we analyze T cell exhaustion by analyzing surface expression of checkpoint inhibitors including PD1 and LAG3. LAG3 expression on T cells within the tumor or chronic viral infection indicate T cell dysfunction. We use this antibody to detect LAG3 expression via flow cytometry.
Flow Cytometry
Chronically infected T cells
1:200 in 2% FBS/PBS for 30 minutes at 4 deg
FC Block 100ng/mL in FACS solution
None
BD FACS Celesta
We are able to detect LAG3 expression on chronically exhausted T cells. They are also PD1 positive indicating good reliable staining.
N/A
Can detect good surface expression of LAG3.
Need to use high dilution.
Great antibody to include in your T cell exhaustion panel. LAG3 is an important marker for such exhaustion.