Cell Signaling Technology (CST)
FoxO1 (C29H4) Rabbit mAb
2880
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Our lab studies the role of mTOR signaling in T cell such as mTORC1 and mTORC2 signaling during T cell differentiation and function. With immunoblot analysis, we assess mTORC2 activity based on Foxo signaling in WT T cells upon T cell activation. When we assess phospho-Foxo1(S256) activity, we also want to determine total Foxo1 to normalize activity to total protein.
Western Blot
Murine T cells
1:3000 in 4%BSA/TBST 4 deg overnight with shaking
5% dry milk/TBST 1 hr room temperature with shaking
1:5000 anti-Rabbit HRP (CST) in %5 milk/TBST 1 hr room temp with shaking
None
UVP Imaging System
We are easily able to detect total Foxo1 levels in our cell lysates. Even better since the pFoxo1 bands fall in the same area as the total Foxo1 bands.
N/A
Very strong and reliable antibody. Can use diluted antibody multiple times if stored properly.
Wonderful antibody to detect total Foxo1 protein levels with western blotting.