Cell Signaling Technology (CST)
Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb
2855
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Our lab studies the role of mTOR signaling in T cell such as mTORC1 signaling during T cell differentiation and function. With immunoblot analysis, we assessed p4ebp1 (T37/46) activity as a direct readout of mTORC1 signaling in WT T cells upon T cell activation.
Western Blot
Activated murine T cells
1:2000 in 4%BSA/TBST 4 deg overnight with shaking
5% dry milk/TBST 1 hr room temperature with shaking
1:5000 anti-Rabbit HRP (CST) in %5 milk/TBST 1 hr room temp with shaking
None
UVP Imaging System
We are able to detect differential p4Ebp1 activity in our activated T cells. There are multiple bands since the antibody can have cross-reactivity to 4EBP2 depending on cell type but generally you are able to determine activity if have unstimulated conditions.
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Make sure you do not run the gel too far long since proteins are small.
Awesome antibody to detect 4EBP1 activity.
Can have some cross reactivity.
Great antibody to assess mTORC1 activity for western blotting. Also can be used for flow cytometry analysis if used with right reagents.