Cell Signaling Technology (CST)
S6 Ribosomal Protein (5G10) Rabbit mAb
2217
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Our lab studies the role of mTOR signaling in T cell such as mTORC1 and mTORC2 signaling during T cell differentiation and function. With immunoblot analysis, we assess mTOR activity in WT T cells upon T cell activation. We use this antibody to normalize total ribosomalS6 protein compared to pS6 (S235.236) activity in certain experiments.
Western Blot
Murine CD8+ T cells
1:10000 in 4% BSA/TBST 4 deg overnight with shaking
5% dry milk/TBST
1:5000 anti-Rabbit HRP (CST) in 5% Milk/TBST 1hr Room Temp with shaking
None
ECL and UVP Imaging System
We are able to detect total ribosomal S6 levels in our lysates. The signal is quite strong depending on experimental conditions.
N/A
Dilute appropriately
Reliable and robust antibody for ribosomal S6.
None.
Great antibody to detect total ribosomal S6 protein when conducting western blots for mTOR signaling.