Cell Signaling Technology
Phospho-Akt (Ser473) (D9E) XP Rabbit
4060
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Our lab studies the role of mTOR signaling in T cell such as mTORC2 signaling during T cell differentiation and function. With immunoblot analysis, we assessed mTORC2 activity using pAKT (S473) as a readout in WT T cells upon T cell activation.
Western Blot
Murine T cells
1:2000 in 4%BSA/TBST
5% Milk/TBST
1:5000 anti-Rabbit HRP (cell signaling) in 5%Milk/TBST
None
UVP Imaging System
We are able to detect mTORC2 activity by assessing pAKT (S473) in activated WT T cells and proving specificity by using PP-242 to inhibit mTOR signaling.
N/A
Very reliable clean antibody without any noise.
Great antibody to assess mTORC2 activity with immunoblot analysis. Most widely used antibody for mTORC2 signaling.