Quantification of BDNF Post-Transfection in Primary Neuronal Cells

April 26, 2019

North Dakota State University
1401 Albrecht Blvd. Sudro 136
Graduate Student

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Company:

Boster Biological

Product Name:

Human BDNF PicoKine™ ELISA Kit

Catalog Number:

EK0307

The kit was used to quantify amount of BDNF produced after transfection with plasmid DNA encoding human BDNF using non viral vector, liposomes.

Experimental Design and Results Summary

Application

Quantification of BDNF in cell lysate and culture medium

Starting Material

Cell lysate and culture medium

Tips

Don't dilute sample, assay directly

Results Summary

Provided good reliable quantification of BDNF produced. No interference. Good reproducibility.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Reliable estimation, good sensitivity

The Bad

Long assay time

The Bottom Line

Would purchase again. Provided good reproducible results. Reasonable price as well.

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