Boster Biological
Mouse Adiponectin PicoKine™ ELISA Kit
EK0596
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I used this kit to quantify adiponectin adipokine in cell culture supernatant (3T3-L1 cells) and mouse serum (C57BL6 mice on control/ western diet). The goal was to check if western diet treatment reduced serum and tissue adiponectin levels.
Adiponectin quantification and tissue and serum
Serum, tissue homogenate
Dilute tissue homogenate 1:100, use serum directly
The results were reasonable and reproducible. Although we had to use two different standard curves for high and low concentration estimations. Performing a standard curve with each sample helped.
N/A
Reliable estimation, good supplier, reasonable price
Long assay time (but was worth the results for me)
Will keep using it. Very good estimation and good price for the kits.