Abcam
Anti-BTK (phospho Y551) antibody [EP267Y] (ab40770)
ab40770
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In our lab, we aim to study a small molecule kinase inhibitor’s activities in various cell lines by investigating phosphor-proteins of interest expression levels. This antibody that we used is good for western blotting p-BTK Y551. It is reliable, easy to use, and does not need much optimization.
Western Blot
Ramos Cell lysates
1:1000, overnight, four degrees Celsius
Licor blocking solution
1:5000, 2 hours, room temp
None
Fluorescence imaging
When Ramos cells were stimulated with anti-IgM (10ug/mL) for 10 minutes, the p-BTK Y551 signal increases as expected, shown by western blot. This is a reliable antibody for Western blot applications.
N/A
Easy to use, reliable results
This is a reliable antibody for Western blot applications.