Agilent Technologies
QuikChange II XL Site-Directed Mutagenesis Kit
200521
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Used it several times for introducing point mutations. Works efficiently even in large vectors. I have used vectors up to 6kb. Very few false positive colonies and most clones do not have unwanted mutations either. Hands-on time is very minimum and very easy to use.
Site Directed Mutagenesis
DNA
Easy to use, efficient and very low number of false positive clones
N/A
Low number of false positive colonies
None
Absolutely recommended for site-directed mutagenesis!