Anti-fl-Notch1 Antibody (CST3608)

April 04, 2019

Surgery
University of Pittsburgh
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Anti-fl-Notch1 antibody

Catalog Number:

3608

Ad-iNOS significantly increased activated TACE and Hes1 expression in CD24+CD133+ PLC/PRF/5 cells, and the increases were attenuated by TAPI-2 (20 μM or 50 μM, 6 h) in a dose-dependent manner.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cell lysates

Primary Incubation

1/1000

Blocking Agent

5% milk

Secondary Incubation

1/2000

Tertiary Incubation

Room temperature 1 hour

Detection

ECL

Results Summary

Ad-iNOS significantly increased activated TACE and Hes1 expression in CD24+CD133+ PLC/PRF/5 cells, and the increases were attenuated by TAPI-2 (20 μM or 50 μM, 6 h) in a dose-dependent manner.

DOI or PMID #

https://doi.org/10.1073/pnas.1722100115

Additional Notes

Fig. 4C

Image Gallery

Summary

The Good

Worked well

The Bad

N/A

The Bottom Line

This antibody worked well to detect the full-length of Notch 1 (300 kDa) and the cleaved one (120 kDa).

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