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FITC anti-mouse CD19 Antibody
115506
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Blood was drawn from an 8 week old naive B6 mouse. PBMC was isolated following histopaque gradient. PBMC was then washed with FACS buffer. The cells were treated with FITC anti-mouse CD19 Antibody at a dilution of 1:100 for 20 min on ice. After 20 min incubation the cells were washed 3 times with FACS buffer and was ready for acquisition.
Flow Cytometry
Mouse PBMC
Dilution 1:100, Time: 20 min, Incubation: Ice.
N/A
Using the product directly (FITC conjugated anti-mouse CD19 Antibody)
About 40% of the total mouse PBMC was identified as CD19+ B cells
Very good FACS antibody. Great separation.
Only for FACS
A good antibody to detect mouse B cells in PBMC and other mouse tissues.