IL-6 Antibody from Novus Biologicals

Institute of Genetic Medicine
Johns Hopkins University
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Novus Biologicals

Product Name:

IL-6 Antibody

Catalog Number:

NBP2-16957

Clone Number:

My research is focused on the response of human cancer cells, specifically breast cancer cells, to different drugs, and the molecular mechanisms underlying their response. This antibody was used to detect IL-6 expression in breast cancer cell lines in response to IL-6 neutralizing antibody by western blot analysis.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human breast cancer cell line

Primary Incubation

1:500

Blocking Agent

5% milk

Secondary Incubation

1:1000

Tertiary Incubation

N/A

Detection

Enhanced chemiluminescence detection kit

Results Summary

This antibody detects IL-6 expression in breast cancer cell lines.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Signal is specific.

The Bad

Signal is not very strong and may need increase for longer time

The Bottom Line

This antibody detects IL-6 expression in breast cancer cell lines.

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