Novus Biologicals
P2Y12/P2RY12 Antibody
NBP2-33870
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The aim of our research is to study neuroinflammation in animal models with neurodegenerative disease. The specific goal of this experiment was to quantify microglial activation in the course of disease progression. This antibody provided excellent staining for optic nerve microglia.
Immunofluorescence
Feline optic nerve
Diluted at 1:400, overnight at 4°C
10% normal donkey serum + 0.5% triton X + 0.1M PBS
Alexa 488 conjugated anti-rabbit IgG 1:500, 1 hr at RT
N/A
Fluorescence
Clearly stains microglia in feline optic nerve tissues.
Robust staining with no unspecific binding
None
Highly recommended for microglia detection in central nerve system.