Cell Signalling Technology
p21 Waf1/Cip1 antibody
2947
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Currently, there are no targeted therapies for triple negative breast cancer patients. Our lab focused on identifying novel molecules to develop targeted therapies for triple negative breast cancer patients. Our research program is involved in characterizing protein kinases and transcription factors as therapeutic targets for these patients. We used this antibody in western blot to detect p21 protein.
Western Blot
Protein sample from human cancer cells
Antibody was diluted at 1:1000 in 5% milk for overnight at 4°C on shaker
5% milk in 0.01% TBS-T
Anti-rabbit IgG conjugated with HRP diluted at 1:3000 for 1 hour at room temperature on shaker
N/A
ECL and Film
To detect wild-type p53 activity, we measured p21 levels in p53 (wild-type) overexpressed cells. This antibody produced bands for p21.
Good antibody
Nothing
Recommend this antibody to measure p53 WT activity