Great for ELISAs from Macrophage Cells

Duke University
Pharmacology
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

TNFa Human ELISA Uncoated

Catalog Number:

88-7346-86

THP-1 cells were treated with LPS for 24 hours and treated with a TAK1 inhibitor. The ELISA kit was able to show TNFa levels in cell culture.

Experimental Design and Results Summary

Application

ELISA

Starting Material

Human macrophages culture media

Protocol Overview

Works well for TNFa created from macrophages.

Tips

Optimize appropriate dilutions from samples for optimal detection.

Results Summary

Worked great at 1:10 dilution and gave fast reliable results.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Fast/sensitive

The Bad

None noted

The Bottom Line

Works great for sensitive TNFa detection.

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