Tocris Bioscience
MTT
5224/500
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Cell metabolism and cell viability are important tools in various cell culture studies.
Cell culture
HL-1 cells
HL-1 cells were treated with 15d-PGJ2 (15 μM) for 0-12 h followed by incubation with MTT (0.5 mg/ml; dissolved in serum-free medium) for 30 min at 37°C. The converted insoluble dark purple coloured dye (formazan) was solubilized with 300 µl acidic isopropanol (0.04 M HCl in absolute isopropanol).
N/A
Cell proliferation was assessed by measuring optical densities at emission and correction wavelengths of 570 and 630 nm, respectively, on a microtiter plate reader.
Worked as expected
MTT can be used to investigate cell metabolism and viability.