Single-Cell Suspension of Whole Lung Tissue from Rats

Boston University
Center for Regenerative Medicine
Postdoctoral Associate

Overall

Performance

Ease-of-Optimization

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Company:

Worthington Biochemical Corporation

Product Name:

Collagenase Type IV

Catalog Number:

LS004186

The aim of this experiment was to digest rat whole lung tissue using Collagenase Type IV. The reagent was recommended by colleagues.

Experimental Design and Results Summary

Application

Tissue digest

Protocol Overview

Incubate minced rat whole lung in collagenase Type IV at 37°C.

Tips

Optimize digestion time.

Features Summary

None

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Single-cell suspension derived using this digestion method.

The Bad

None

The Bottom Line

The digestion time needs to be adjusted as per requirement.

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