BioLegend
Anti-mouse CD279 APC
135209
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This antibody was used to stain for CD279 (PD-1) expressing cells in mouse splenocytes stimulated with PMA/ionomycin. It was chosen because it is a commonly used clone for CD279, APC is bright and works well with other fluorophores in the staining panel, and BioLegend had a competitive price.
Flow Cytometry
Mouse splenocytes
1:100 dilution in 100 µL total staining volume for 15 minutes at room temperature.
FcBlock + 2% FBS
N/A
BD LSR II
CD279 staining was excellent (filled histogram) as compared to unstained cells (dotted line).
Works well. Reproducible results. Low price.