BioLegend
Anti-mouse HVEM APC
136305
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This antibody was used to stain for CD270 (HVEM) in mouse splenocytes. It was chosen because it is a commonly used clone for CD270, APC is bright and works well with other fluorophores in the staining panel, and BioLegend had a competitive price.
Flow Cytometry
Mouse splenocytes
1:100 dilution in 100 µL total staining volume for 15 minutes at room temperature.
FcBlock + 2% FBS
N/A
BD LSR II
HVEM staining was excellent (filled histogram) as compared to unstained cells (dotted line).
Works well. Reproducible results. Low price.
Excellent staining and reproducible results. Will continue to use this antibody.