BioLegend
Anti-mouse B7-H1 PE
124307
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This antibody was used to stain B7-H1 (PD-L1)-expressing cells in mouse splenocytes. It was chosen because it is a commonly used clone for B7-H1, PE is bright and works well with other fluorophores in the staining panel, and BioLegend had a competitive price.
Flow Cytometry
Mouse splenocytes
1:100 dilution in 100 µL total staining volume for 15 minutes at room temperature.
FcBlock + 2% FBS
N/A
BD LSR II
B7-H1 staining was excellent (filled histogram) as compared to isotype control (dotted line).
Works well. Reproducible results. Low price.
Will continue to use this antibody.