PE/Dazzle™ 594 CD19 Antibody to Detect B Cells in Mice

February 15, 2019

Microbiology and Immunology Department
Emory University
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE/Dazzle™ 594 anti-mouse CD19 Antibody

Catalog Number:

115554

This antibody was used to stain B cells in spleen, blood, liver, lung, and bone marrow.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Spleen

Primary Incubation

Antibody dilution: 1:100, Incubation time: 20 min, Temperature: On ice

Blocking Agent

Fc block (TruStain FcX) for 5 min

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

PE/Dazzle 594 anti-mouse CD19 Antibody

Results Summary

The antibody is excellent for staining B cells. The example shown here is spleen but works perfectly for other tissues too. Liver, lung, and bone marrow are the other tissues tested. Works great in staining PBMC B cells too.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Great Ab for FACS. The bright Pe/Dazzle makes the 6D5 clone brighter.

The Bad

Can be used only in flow cytometry

The Bottom Line

A very bright Ab for FACS

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