Bright Staining of TCRgd

January 16, 2019

Center for Infectious Medicine
Karolinska Institute
Postdoctoral Researcher

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Company:

Miltenyi Biotec

Product Name:

Anti-human TCRgd-FITC

Catalog Number:

130-113-511

PBMC of a healthy donor were stained for viability dye and anti-human TCRgd-FITC to detect TCRgd-expressing cells. The antibody has great resolution and is bright.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

PBMC

Primary Incubation

15 min at room temperature (titer 1:50)

Blocking Agent

Included in staining buffer (PBS containing EDTA and 2% FCS)

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry (BD LSRFortessa)

Results Summary

Bright staining on PBMC enables clear detection of TCRgd-expressing cells after gating on single viable lymphocytes.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Great resolution

The Bad

None

The Bottom Line

Would recommend!

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