Excellent Immunostaining of Cortical Neurons

pediatrics
University of Mississippi Medical Center
Assistant Professor

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology

Product Name:

SATB2 (SATBA4B10) Antibody

Catalog Number:

sc-81376

The general aim of our research is to study neurogenesis in rodents with maternal immune activation. The specific goal of this experiment was to quantify proliferation of neural progenitor cells and migration of immature neurons during corticogenesis. This antibody provided excellent staining for cortical immature neurons.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Free-floating brain sections from P1 rat or mice

Primary Incubation

Satb2 diluted at 1:400, overnight at 4°C

Blocking Agent

10% serum + 0.5% triton

Secondary Incubation

Biotin anti-mouse IgG 1:400, 1.5 hr at RT

Tertiary Incubation

Avidin-Alex488 1:400

Detection

Fluoresence

Results Summary

Clearly stains cortical neurons in developing rat or mice brain

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Clear staining with very low background

The Bad

None

The Bottom Line

Highly recommended

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