FB Essence: A Cost-Effective, Quality Alternative to FBS

Case Western Reserve University
Urology
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

VWR

Product Name:

VWR Life Science Seradigm FB Essence

Catalog Number:

10803-034

We have been using FB Essence from VWR (VWR Life Science Seradigm FB Essence) since late 2016 and found it to be a very viable alternative to Fetal Bovine Serum (FBS). It is very cost effective and could be used to grow a variety of mammalian cancer cells, both suspension and adherent cell. We have used the FB essence for growing both adherent and suspension cells that are a little finicky also. They grow well and have provided us with reproducible results and the FBS essence does not affect or change the morphology of the cells. We have made comparisons between the FBS and the essence and found them to be very similar. FBS essence is 100% US origin and very cost effective. I would recommend scientists to try them. We have also mixed them with FBS from various companies and Seradigm and have used them too. We have found our cells going very well. We have observed them to as good as the ones growing in FBS. The Service from VWR and Seradigm is excellent both technical and customer. In a scenario where grant money is very slim, VWR and Seradigm have come out with a quality, cost-effective product for research.

Experimental Design and Results Summary

Application

Cell Culture

Starting Material

Mammalian Cell Culture

Protocol Overview

Basic mammalian cell culture protocol followed in all labs.

Tips

The FBS essence can be mixed with regular FBS in 1:1 ratio. The cells grow really well.

Results Summary

A good product that provides good results and is very cost effective. The morphologies and phenotype are not affected. The characteristics are maintained.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

The product could be used directly and also could be mixed with various brands of FBS for use. We mix them half and half with FBS and they give the best results.

The Bad

Nothing so far.

The Bottom Line

A quality cost-effective product that is a good substitute for FBS which is turning out to be very expensive.

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