Good Antibody for Siglec-1 Detection on Tissue Sections

January 16, 2019

Neuroscience
Wisconsin University
Postdoctoral Researcher

Overall

Quality of Results

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Company:

Novus Biologicals

Product Name:

Siglec-1/CD169 Antibody

Catalog Number:

NB600-534

This antibody was used in a study that investigated inflammatory status in small intestine. The antibody reviewed here provided reasonable results in Immunofluorescence staining of Siglec1.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Fixed frozen mouse small intestine tissue section

Primary Incubation

1:500

Blocking Agent

Normal donkey serum

Secondary Incubation

Alexa fluor 568 conjugated donkey anti-mouse antibody

Tertiary Incubation

N/A

Detection

AF 568 channel with epifluorescence microscope

Results Summary

This antibody worked excellently in immunofluorescence on tissue sections, shown as target-red and DAPI-blue. Only minimal background staining compared to isotype control could be detected. The antibody highlights the presence of macrophages in tissues.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

No antigen retrieval needed. High specificity.

The Bad

None

The Bottom Line

This is a very good antibody to detect Siglec1 on mouse tissue. The minimal background staining is negligible when comparing to negative controls.

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