Time Course Effect Of Chemoattractant On H2A.Xs Phosphorylation

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

GeneTex

Product Name:

Histone H2A.XS139ph (phospho Ser139) antibody [3F2]

Catalog Number:

GTX80694

Histones are basic nuclear proteins that maintains the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. Here we checked the time course effect of chemoattractant on H2A.Xs phosphorylation.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse lysate

Primary Incubation

1:1000 dilution prepared in 1x TBST

Blocking Agent

5% BSA prepared in 1x TBST

Secondary Incubation

1:4000 dilution prepared in 1x TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

There is a time dependent increase in H2A.XS phosphorylation by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

This antibody is very good for western blot experiment

The Bad

Background noise is more

The Bottom Line

This antibody works great for western blot but the background is more. Highly recommended.

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