Invitrogen
Cholera Toxin Subunit B (Recombinant), Alexa Fluor™ 555 Conjugate
C34776
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This reagent is used to stain the GM1 of the cell membrane and subsequently used for fluorescence microscopy imaging to see if there is any changes in the cell morphology upon a change in the stimulus. The product is easy to use and specific.
Fluorescence Microscopy
AGS gastric cancer cell line
(1) Stain is diluted in the cell medium. (2) Cell medium is replaced with the new medium containing the dye. (3) Staining is incubated at 4°C for one hour.
Dye has strong binding, recommended to carry out dilution prior to staining.
The results produced are sensitive and specific. Thus, there isn't much background noise observed when the image is taken.
N/A
Easy to use and it is specific.
NIL
A highly recommended dye for those who are interested in staining the GM1 of cell membrane.