The Antibody Stains PD-1+ Cells Well

Pathology
Stanford University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Alexa Fluor® 647 anti-mouse CD279 (PD-1) Antibody

Catalog Number:

135230

We wanted to study T cells in the diseased and healthy state, so we used the antibody to stain PD-1+ T cells in the lymph nodes. The antibody works well and is clear.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of mouse mesenteric lymph node cells

Primary Incubation

1:200

Blocking Agent

FcBlock

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSRII flow cytometer

Results Summary

The antibody identifies the PD-1+ T cell population very clearly.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

The antibody stains PD-1 very well.

The Bad

None to mention

The Bottom Line

The antibody is great for identifying PD-1+ T cells.

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