The Antibody Stains CXCR5+ Cells in the Lymph Nodes Very Well

January 15, 2019

Pathology
Stanford University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

FITC anti-mouse CD185 (CXCR5) Antibody

Catalog Number:

145520

We wanted to study CXCR5+ cells in the diseased vs healthy state, so we used this antibody to identify CXCR5+ cells in the lymph node. The antibody is clear and works well.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of mouse mesenteric lymph node cells

Primary Incubation

1:200

Blocking Agent

FcBlock

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSRII flow cytometer

Results Summary

The antibody identifies the CXCR5+ lymphocyte population very clearly.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

The antibody stains CXCR5+ cells in the lymph nodes very wel.l

The Bad

None to mention

The Bottom Line

The antibody is great for identifying CXCR5+ lymphocytes.

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