The Antibody Identifies CD4+ Cells Very Well

Pathology
Stanford University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 785™ anti-mouse CD4 Antibody

Catalog Number:

100552

We wanted to study CD4+ T cells in the diseased vs healthy state, so we used the antibody to stain CD4+ T cells in the lymph nodes. The antibody is clear and works well.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of mouse mesenteric lymph node cells

Primary Incubation

1:200

Blocking Agent

FcBlock

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSRII flow cytometer

Results Summary

The antibody stained the CD4+ T cell population very clearly.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The antibody stains the CD4+ T cell population clearly.

The Bad

None to mention

The Bottom Line

The antibody is great for identifying CD4+ T cells.

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