The Antibody Identifies CD21+ B Cells Very Well

January 07, 2019

Pathology
Stanford University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

APC anti-mouse CD21/CD35 (CR2/CR1) Antibody

Catalog Number:

123412

We wanted to study B cells in the disease vs healthy state, so we used the antibody to identify CD21+ B cells in the spleen. The antibody is great to identify marginal zone B cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of mouse spleen cells

Primary Incubation

1:200

Blocking Agent

FcBlock

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSRII flow cytometer

Results Summary

The antibody identifies CD21+ marginal zone B cells very clearly.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The antibody identifies marginal zone B cells very well.

The Bad

None to mention

The Bottom Line

The antibody is very useful for staining CD21+ cells.

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