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Brilliant Violet 605™ anti-mouse CD279 (PD-1) Antibody
135220
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As a cancer immunology lab, we assess T cell exhaustion by analyzing surface expression of checkpoint inhibitors such as PD1. PD1 expression on T cells within the tumor indicates exhaustion and T cell dysfunction. We use this antibody to detect PD1 expression via flow cytometry. The antibody is good and bright.
Flow Cytometry
Murine T-cells from the tumor
1:500 in FACS buffer
FC block 100 µg/mL in FACS buffer
None
BD Celesta flow analyzer
We are able to detect a distinct PD1 positive population in T cells within the tumor.
N/A
Really good and bright antibody to detect PD1 expression
None whatsoever
Great flow antibody to detect PD1 expression on a relatively unused flow channel (BV605). Very consistent staining over time.