Purified Anti-ß-Amyloid, 17-24 Antibody

Proteostasis in Aging and Disease
Leibniz-Institute of Molecular Pharmacology
Ph.D. Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Purified anti-β-Amyloid, 17-24 Antibody

Catalog Number:

800712

The anti-amyloid beta antibody was used in a western blot to determine if amyloid-beta (42) had been successfully purified after a new purification procedure.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Purified amyloid beta m1–42

Primary Incubation

Purified anti-β-Amyloid, 17-24 Antibody

Blocking Agent

4% BSA in TBST

Secondary Incubation

Anti-mouse antibody

Tertiary Incubation

N/A

Detection

Lumi Imager F1

Results Summary

Amyloid beta m1-42 was passed on a gel before a western blot using anti-β-Amyloid, 17-24 Antibody was performed. Results showed that before HPLC the sample contained not only monomerized fractions of beta but also oligomerized and aggregated fractions.

DOI or PMID #

N/A

Additional Notes

Used at 1/2000 dilution in 4% BSA in TBST

Related Categories

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Summary

The Good

Easily imaged using the Lumi Imager

The Bad

No downsides

The Bottom Line

It worked well for us. I would recommend it for anyone who requires an amyloid beta antibody.

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