Alexa Fluor® 700 Anti-Human CD45 Antibody from Biolegend

January 02, 2019

Innate Immunity
Deutsches Rheuma-Forschungszentrum
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Alexa Fluor® 700 anti-human CD45 Antibody

Catalog Number:

304023

CD45 is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets, and thus can be used to identify immune cells such as T cells, B cells, and innate lymphoid cells. In this panel, we use CD45 to identify immune cells in peripheral blood, in combination with other markers to pinpoint other populations. The antibody is bright and specific.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMCs

Primary Incubation

15 min, 37°c

Blocking Agent

0.5% BSA in PBS

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSRII Fortessa, software FACS Diva

Results Summary

This antibody was found to be bright and specific against human CD45, with high resolution, as tested in human PBMCs, on a panel to identify lymphocytes populations.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Bright, specific, high resolution

The Bad

N/A

The Bottom Line

Great anti-human CD45antibody

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