eBioscience/Invitrogen
eBioscience™ Fixable Viability Dye eFluor™ 780
65-0865-18
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We are interested in studying the mechanism of T cell differentiation through modulating the mTOR pathway. We use this dye to distinguish live cells from dead cells in tissues. It is very bright and works well.
Flow cytometry
Mouse splenocytes
Mouse splenocytes were stained in FACS buffer for 20 minutes along with other antibodies.
Use less dye than protocol suggets
Be able to distinguish between live or dead cells from tissues
N/A
Very bright dye to distinguish dead cells
Must be really careful with the dilution
One of the best live/dead dyes available and provides consistent results