IgG2a Control

December 18, 2018

Institute of Biomedical Engineering
University of Oxford
Ph.D. Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Purified Mouse IgG2a, κ Isotype Ctrl Antibody

Catalog Number:

401501

This product provides a very useful standard control for quantifying total IgG2a antibody concentrations in murine serum.

Experimental Design and Results Summary

Applications

ELISA

Sample

Used as a standard to quantify total IgG2a in mouse serum

Primary Incubation

Overnight to bind to the plate

Blocking Agent

10% FBS in PBS

Secondary Incubation

2 hours in PBS

Tertiary Incubation

1 Hour with anti-IgG1 antibody conjugated to HRP

Detection

TMB Ultra substrate

Results Summary

This product provides a very useful standard control for quantifying total IgG2a antibody concentrations in murine serum.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very useful as a standard and positive control

The Bad

N/A

The Bottom Line

This product provides a very useful standard control for quantifying total IgG2a antibody concentrations in murine serum.

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