Cell Signaling Technology
Phospho-Akt1 (Ser473) (D7F10) XP® Rabbit mAb (Akt1 Specific)
9018
Info:Supplier Page View Product SpecsView Product
Browse Similar Products: Phospho-Akt1 (Ser473) Western Blot Antibodies
Our lab studies the role of mTOR, specifically mTORC2 signaling, in macrophage polarization and function. We assessed mTORC2 activity using pAKT as a readout using WT and Rictor KO macrophages to prove function when we knocked out Rictor. This antibody is easy to use and works well.
Western Blot
Murine macrophages (WT and Rictor KO)
1:2500 in 4%BSA/TBST
5% dry milk/TBST
1:5000 Rabbit IgG HRP in 5%dry milk/TBST
None
Invitrogen ECL Pico
We are able to detect mTORC2 activity by assessing pAKT (S473) in WT macrophages and proving specificity by using Rictor KO macrophages.
28877476
N/A
Great monoclonal antibody to detect mTORC2 signaling
Easy-to-use antibody to analyze mTORC2 activity via western blotting.