Easy Reagent for Assessing Expression of Genes in Human Tissues

December 11, 2018

Princeton
Molecular Biology
Graduate Student

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Company:

Clontech

Product Name:

Human Bone Marrow Total RNA

Catalog Number:

636591

The product contains unmodified RNA from a pooled sample of recently deceased individuals, specifically from the bone marrow. The RNA sample was converted to cDNA using the Invitrogen superscript IV kit, and then qPCR was performed under standard conditions with human primers for CD31, GapDH, and E-selectin. All genes were detected within 24 cycles, indicating high concentration and quality of the sample.

Experimental Design and Results Summary

Application

Quantitative real-time qPCR

Starting Material

N/A

Protocol Overview

N/A

Tips

Use superscript IV kit for cDNA synthesis

Results Summary

Results were very replicable with low variability. Reagent was compared to other pooled RNA samples for informative gene expression levels across human organs.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Works exactly as intended to clone or quantify genes in human tissues

The Bad

Doesn't come as a kit, each RNA is individually bought

The Bottom Line

Combining this reagent with other pooled RNA samples from this and other companies gives a reliable indicator of gene expression in human tissues.

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