Santa Cruz Biotechnology
Dvl-3
sc-8027
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Browse Similar Products: Dvl-3 Western Blot Antibodies
Here we were investigating if a novel cytosolic repressor of the Wnt pathway was causing degradation of Dvl2 or Dvl3. Samples with knockdown or overexpression of this repressor were stimulated with Wnt3a, and then Dvl3 was probed. The results were negative. Antibody staining by traditional western showed expected double band in the correct position; however, the signal was very weak.
Western Blot
Human breast cancer MDA-MB-231
1:500–1:1000 in 5% milk
5% milk in IBS
Licor IRDye800-anti-mouse
None
Licor imaging
Results showed expected doublet band at the correct size with no non-specific bands. Signal was very weak and prevented robust quantification. Traditional HRP detection was not tried.
N/A
Works without non-specific bands
Very low signal/noise
Works if you can't find anything else, but not great