Cell Signaling Technology
PRMT1 (A33) Antibody
2449
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Browse Similar Products: PRMT1 Western Blot Antibodies
Our lab is interested in how glutamine metabolism affects methylation. Since PRMT1 is a post-translational modification, we wanted to look at the expression of the protein after T cells are exposed to varying concentrations of glutamine along with glutamine antagonism. This antibody is faint at times but specific.
Western Blot
Murine T cells
1:5000 in 4°C overnight
5% nonfat dry milk
1:5000 anti-rabbit HRP-linked
N/A
ECL Pico
The signal is a bit faint at times, but it definitely shows a difference in band brightness depending on the condition.
Very specific band on western blot
The dilution might need to be adjusted depending on samples
Very specific band. Definitely works and isn't confusing about where the band will show up