Gibco
Collagenase Type I
17100017
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We always use the collagenase type I specifically for digesting tumor tissue harvested from mice. We are a cell metabolism and immunology lab; therefore, after digesting the tissues and isolating for CD8 cells, we follow up with antibodies for flow cytometry to look at markers specifically for apoptosis, activation markers, memory or effector markers, etc. This product is reliable and we use it regularly.
Flow Cytometry
Murine Tumor
After we extract whole murine tumor tissue from mice, we digest the tumor in RPMI media with 5% FBS, added with collagenase I and DNase. After we incubate in 37°C on a shaker, we filter the tumor tissue on a strainer and proceed with isolating CD8 cells before we stain for flow cytometry.
After incubation, we always add EDTA to inactivate the enzymes.
The collagenase type I works really well in cleaving bonds for tumor tissues. We have reliably used this every single time for our murine tumor harvest, followed by either flow cytometry or sorting cells. It is always extremely clean in terms of our population of interest.
N/A
Easy to use and always reliable
The powder itself is very light, and it's difficult to measure out the weight.
Definitely worth using to get a clean population followed with isolation or Ficoll.