Cell Signaling Technology
Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb
8814
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The WNT pathway is essential for embryonic development and adult tissue homeostasis. Deregulation of this pathway results in cancers of the stomach, intestine, breast, etc. I am interested in studying the molecular mechanism of oncogenic WNT signal transduction to identify potential targets for therapeutic intervention of colon cancer. The goal of this experiment is to determine good working antibodies for the purpose of blotting some of the core components of the WNT pathway. This antibody was clean and didn't give any non-specific bands.
Western Blot
RKO (whole-cell extract)
1:1000 dilution in 1xTBS-T; overnight at 4°C for 16 hr
5% BSA in TBS-T
1:10,000 dilution of donkey anti-rabbit IgG+HRP; 1 hr at room temperature
None
ECL
RKO cells were treated with WNT3A for 0–2 hr and one can see the nice gradual accumulation of active beta-catenin.
N/A
Specific band corresponding to active beta-catenin
Excellent antibody for western blotting