Western Blot Detection of NCS1

November 16, 2018

Biochemistry and Molecular Biology
University of Arkansas for Medical Sciences
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-NCS1 antibody [EPR7699]

Catalog Number:

ab129166

Abcam's rabbit monoclonal antibody to NCS1 works as intended. I was able to detect the predicted protein size in both murine (MEF) and human (293T) cells lines.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Whole-cell lysate from MEF and 293T cell lines (20 µg)

Primary Incubation

1:1000 dilution. 16 hours at 4°C in 1% Milk PBSTween

Blocking Agent

5% milk

Secondary Incubation

Donkey Anti-Rabbit IgG 1:5000 in 1% milk PBSTween

Tertiary Incubation

N/A

Detection

ECL+

Results Summary

This NCS1 specific antibody easily detected the NCS1 protein at ~22 kDa. Clean blot with no non-specific proteins showing up.

DOI or PMID #

N/A

Additional Notes

Very good, clean antibody

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Summary

The Good

Very clean and specific.

The Bad

None

The Bottom Line

Use this antibody for detection of murine and human NCS1.

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