Santa Cruz
LGR4 Antibody (C-12)
sc-390630
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I am interested in studying the molecular mechanism of hedgehog signal transduction in vertebrate cells. I had performed a CRISPR/Cas9 genetic screen to uncover new regulators of hedgehog signaling pathway. One of the negative regulators identified in these screens is known to regulate a cell surface receptor relevant to hedgehog pathway. The goal of this experiment is to determine if this gene affects the overall levels of one of the R-spondin receptors, LGR4.
Western Blot
NIH-3T3 whole-cell extract
1:200 dilution in 1xTBS-T; overnight at 4°C for 16 hr
5% milk in TBS-T
1:10,000 dilution of donkey anti-mouse IgG+HRP; 1 hr at room temperature
None
ECL
The predicted size is 104 kDa and this antibody picks up a band at ~100 kDa.
N/A
Picks up a band at expected size
Few non-specific bands
Good for western blotting