Anti-Mouse CD11b Antibody for Flow Cytometry

November 13, 2018

Advanced Diagnostics
University Health Network
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PerCP/Cy5.5 anti-mouse/human CD11b

Catalog Number:

101227

Identification of cardiac myeloid cells with anti-mouse CD11b antibodies

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Cardiac tissue

Primary Incubation

15 minutes

Blocking Agent

Anti-CD16/CD32 antibody

Secondary Incubation

30 minutes

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

Anti-mouse CD11b antibody in combination with other more specific markers helps identify cardiac myeloid cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

PerCP/Cy5.5 anti-mouse CD11b identifies cardiac myeloid cells.

The Bad

It does not help in subset identification.

The Bottom Line

PerCP/Cy5.5 anti-mouse antibody identifies myeloid cells in cardiac tissue by flow cytometry.

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