Robust and Reliable Antibody to Identify KLRG1+ Terminal Effector CD8 T Cells

November 12, 2018

Pharmacology and Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher (eBioscience)

Product Name:

KLRG1 Monoclonal Antibody (2F1), PE-eFluor 610

Catalog Number:

61-5893-82

Our lab studies CD8 T cell memory differentiation. KLRG1 is a readily used marker to identify terminal effector CD8+ T cells. During analysis of CD8 T cells in an acute response, I used this antibody in my T cell panel to identify memory precursors from terminal effector T cells. Based on years of use of this particular clone, we always stick with Thermo (eBio) since they are one of the few that carry it.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine Splenocytes

Primary Incubation

1:1000

Blocking Agent

FC Block (10ug/ml)

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD Celesta

Results Summary

I can easily readily identify KLRG1+CD8+ T cells from the spleen defined as terminal from the memory precursors during an acute infection.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Very reliable antibody to identify KLRG1+ cells. 1:1000 dilution certainly helps with cost.

The Bad

The flourochrome is a bit hard to compensate but can be done.

The Bottom Line

Awesome antibody at a great price since we use it very diluted. Perfect way to identify terminal effector T cells.

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