Great Thy1.1 Congenic Antibody

November 09, 2018

Pharmacology and Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Alexa Fluor 700 anti-rat CD90/mouse CD90.1 (Thy-1.1) Antibody

Catalog Number:

202528

Our lab studies the role of mTOR signaling and metabolism in CD8 T cell differentiation and function. We perform numerous adoptive transfer experiments of donor transgenic T cells (CD90.1) into different WT B6 congenic hosts (CD90.2). To analyze the donor T cells, we use CD90.1 to distinguish host versus donor. This marker is quite robust since it separates quite well from the host CD8 T cells. The AF700 Thy1.1 antibody readily fits into my panel since it's usually a dim channel, but this antibody is quite too bright. We are able to readily identify the donor cells using this antibody.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine Splenocytes

Primary Incubation

1:2000

Blocking Agent

FC Block (CD16/32) 10 µg/mL

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD FACS Celesta

Results Summary

I analyzed the adoptively transferred cells (CD90.1) during the acute response of a co-adoptive transfer experiment of two populations: CD90.1/2 and CD90.1/1.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Reliably good and bright at such a dilute concentration so it's cheaper in the long run

The Bad

Must titrate the antibody since it's too bright

The Bottom Line

Truly a great antibody to fit into the Red channel of a multi-color flow panel. This antibody is quite bright to identify the donor cells.

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